Fig. 2. ICV injection of PNU reduces food intake in the male mouse. PNU (450 picomol/mouse) was administered by ICV injection; after 40 minutes the mouse was submitted to a respirometric evaluation of (A) heat, (B) eating time, and food intake in 1 h (C), 2 h (D), 4 h (E), 8 h (F) and 24 h (G). (saline n = 5, PNU n = 4). * p <0.05 ** p <0.01. HYPO= Hypothalamus.